Decrease the amount from µL to 50 µL.Īdditionally, you should give DNA time to dissolve after the extraction. If you need high concentrations of DNA you can increase the concentration by removing the amount of buffer which is used to dissolve the DNA. You have to incubate it only once, before you remove an aliquot for DNA extraction. It is very important that the incubation is carried out in the original sample tube. Remember that you should use a very tiny amount, so that the donor can’t even taste it. Adding some sugar on the tongue can also help with that. One of them is by rubbing the cheeks, behind the back teeth. If someone is unable to spit the 2 ml which are required, there are ways to stimulate the saliva production. This way you will be able to collect all the saliva you need and things will be easier for your donor as well. In this situation you must use a kit such as OG-575 which was designed specifically for non-spitters. For example, if you’re are collecting it from a small child, you can’t tell him or her to spit. In some cases, it might be difficult to collect all the saliva you need. Only with the right amount of saliva the tests can be accurate. The liquid is the one that must reach the limit, and bubbles should be somewhere above it. Testing samples from different donors is required in order to get a clear idea about the yields that you should prepare for.Īdditionally, saliva is the one that must reach the “fill to” line. When it comes to yields, you should remember that they are different based on the donor. If the donors do not respect the indications, the DNA might not lead to accurate results.
The donors must deliver the saliva without eating, drinking or smoking for 30 minutes prior to giving the sample. It is vital that you follow the instructions carefully. While collecting the samples you must be extra careful.
We have put together the best tips from experts so that the dna testing kit results are as accurate as possible.
However, processing the sample is the important step, and there are a couple of things which can be done in order to make sure that you get accurate results. DNA tests require only a saliva sample, which is generally not too complicated.
0 kommentar(er)
